SubtiBank SubtiBank
Version comparison:

2019-08-01 13:24:382025-08-08 10:27:18

The protein

Catalyzed reaction/ biological activity

ATP L-glutamate NH3 = ADP phosphate L-glutamine (according to Swiss-Prot)

ATP + L-glutamate + NH4+ --> ADP + H+ + L-glutamine + phosphate (according to UniProt)

The protein

[SW|Cofactors]

Mg(2 )

Mg2+

The protein

Structure

[PDB|4LNN] (apo-GS) [Pubmed|24158439]

[PDB|3QAJ] (complex with ATP)

[http://pdb.org/pdb/search/structidSearch.do?structureId=4s0r 4S0R] (the [[protein|TnrA]]-[[protein|GlnA]] complex) [Pubmed|25691471]

[PDB|A general discussion of GS structure]

[PDB|3QAJ] (complex with ATP)

[PDB|4LNN] (apo-GS) [Pubmed|24158439]

[http://pdb.org/pdb/search/structidSearch.do?structureId=4s0r 4S0R] (the [[protein|TnrA]]-[[protein|GlnA]] complex) [Pubmed|25691471]

[PDB|A general discussion of GS structure]

Biological materials

Mutant

GP247 (''glnA::cat''), available in [SW|Jörg Stülke]'s lab

BKE17460 (''glnA''::''ermC'') (available at the [http://www.bgsc.org/ BGSC] and in [SW|Jörg Stülke]'s lab) [pubmed|28189581]

GP2263 (''glnA''::''ermC'') (available in [SW|Jörg Stülke]'s lab)

BP148 (del(''[[gene|glnR]]-[[gene|glnA]]'')::''cat''), available in [SW|Fabian Commichau]'s lab

GP1883 (del(''[[gene|glnR]]-[[gene|glnA]]'')::''ermC''), available in [SW|Fabian Commichau]'s and [SW|Jörg Stülke]'s labs

BKE17460 ([[gene|glnA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE17460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGGTAAAATTCCTCCT, downstream forward: _UP4_TAATATCTCAATCCCTTGGC

BKK17460 ([[gene|glnA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK17460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGGTAAAATTCCTCCT, downstream forward: _UP4_TAATATCTCAATCCCTTGGC

GP247 (''glnA::cat''), available in [SW|Jörg Stülke]'s lab

BKE17460 (''glnA''::''ermC'') (available at the [http://www.bgsc.org/ BGSC] and in [SW|Jörg Stülke]'s lab) [pubmed|28189581]

GP2263 (''glnA''::''ermC'') (available in [SW|Jörg Stülke]'s lab)

BP148 (del([[gene|glnR]]-[[gene|glnA]])::''cat''), available in [SW|Fabian Commichau]'s lab

GP1883 (del(''[[gene|glnR]]-[[gene|glnA]]'')::''ermC''), available in [SW|Fabian Commichau]'s and [SW|Jörg Stülke]'s labs

BKE17460 ([[gene|glnA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE17460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGGTAAAATTCCTCCT, downstream forward: _UP4_TAATATCTCAATCCCTTGGC

BKK17460 ([[gene|glnA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK17460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGGTAAAATTCCTCCT, downstream forward: _UP4_TAATATCTCAATCCCTTGGC

Biological materials

Expression vectors

expression/ purification from ''E. coli'', with N-terminal Strep-tag (in [SW|pGP172]): pGP174, available in [SW|Jörg Stülke]'s lab

pGP177 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pBQ200]), available in [SW|Jörg Stülke]'s lab

pGP174: expression/ purification from ''E. coli'', with N-terminal Strep-tag (in [SW|pGP172]): , available in [SW|Jörg Stülke]'s lab

pGP177: N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pBQ200], available in [SW|Jörg Stülke]'s lab

pGP2832: N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP380], available in [SW|Jörg Stülke]'s lab