2019-08-01 13:24:382025-08-08 10:27:18
The protein
Catalyzed reaction/ biological activity
ATP L-glutamate NH3 = ADP phosphate L-glutamine (according to Swiss-Prot)
ATP + L-glutamate + NH4+ --> ADP + H+ + L-glutamine + phosphate (according to UniProt)
The protein
[SW|Cofactors]
Mg(2 )
Mg2+
The protein
Structure
[PDB|4LNN] (apo-GS) [Pubmed|24158439]
[PDB|3QAJ] (complex with ATP)
[http://pdb.org/pdb/search/structidSearch.do?structureId=4s0r 4S0R] (the [[protein|TnrA]]-[[protein|GlnA]] complex) [Pubmed|25691471]
[PDB|A general discussion of GS structure]
[PDB|3QAJ] (complex with ATP)
[PDB|4LNN] (apo-GS) [Pubmed|24158439]
[http://pdb.org/pdb/search/structidSearch.do?structureId=4s0r 4S0R] (the [[protein|TnrA]]-[[protein|GlnA]] complex) [Pubmed|25691471]
[PDB|A general discussion of GS structure]
Biological materials
Mutant
GP247 (''glnA::cat''), available in [SW|Jörg Stülke]'s lab
BKE17460 (''glnA''::''ermC'') (available at the [http://www.bgsc.org/ BGSC] and in [SW|Jörg Stülke]'s lab) [pubmed|28189581]
GP2263 (''glnA''::''ermC'') (available in [SW|Jörg Stülke]'s lab)
BP148 (del(''[[gene|glnR]]-[[gene|glnA]]'')::''cat''), available in [SW|Fabian Commichau]'s lab
GP1883 (del(''[[gene|glnR]]-[[gene|glnA]]'')::''ermC''), available in [SW|Fabian Commichau]'s and [SW|Jörg Stülke]'s labs
BKE17460 ([[gene|glnA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE17460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGGTAAAATTCCTCCT, downstream forward: _UP4_TAATATCTCAATCCCTTGGC
BKK17460 ([[gene|glnA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK17460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGGTAAAATTCCTCCT, downstream forward: _UP4_TAATATCTCAATCCCTTGGC
GP247 (''glnA::cat''), available in [SW|Jörg Stülke]'s lab
BKE17460 (''glnA''::''ermC'') (available at the [http://www.bgsc.org/ BGSC] and in [SW|Jörg Stülke]'s lab) [pubmed|28189581]
GP2263 (''glnA''::''ermC'') (available in [SW|Jörg Stülke]'s lab)
BP148 (del([[gene|glnR]]-[[gene|glnA]])::''cat''), available in [SW|Fabian Commichau]'s lab
GP1883 (del(''[[gene|glnR]]-[[gene|glnA]]'')::''ermC''), available in [SW|Fabian Commichau]'s and [SW|Jörg Stülke]'s labs
BKE17460 ([[gene|glnA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE17460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGGTAAAATTCCTCCT, downstream forward: _UP4_TAATATCTCAATCCCTTGGC
BKK17460 ([[gene|glnA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK17460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGGTAAAATTCCTCCT, downstream forward: _UP4_TAATATCTCAATCCCTTGGC
Biological materials
Expression vectors
expression/ purification from ''E. coli'', with N-terminal Strep-tag (in [SW|pGP172]): pGP174, available in [SW|Jörg Stülke]'s lab
pGP177 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pBQ200]), available in [SW|Jörg Stülke]'s lab
pGP174: expression/ purification from ''E. coli'', with N-terminal Strep-tag (in [SW|pGP172]): , available in [SW|Jörg Stülke]'s lab
pGP177: N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pBQ200], available in [SW|Jörg Stülke]'s lab
pGP2832: N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP380], available in [SW|Jörg Stülke]'s lab